1ADG (Hort. Sci.), KAB-II, ICAR, New Delhi, 110012
2Division of Fruits and Horticultural Technology, IARI, New Delhi, 110012
3Division of Plant Physiology, IARI, New Delhi, 110012
Division of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, New Delhi, 110 012
*Corresponding author's E-mail: mrlegha@outlook.com
Online published on 12 October, 2015.
To establish a resourceful tissue culture system to induce anthocyanin pigments, research was conducted to obtain high quality callus from leaf explant of Rosa hybrida cv. Pusa Ajay. Of the different treatments employed for callus induction using Murashige and Skoog (MS) medium, a treatment combination of 19.60 μM indole3-butyric acid (IBA) + 4.65 μM kinetin (Kin) + 108.6 mM adenine sulphate (AdS) gave the maximum induction coefficient (97.75 ± 1.11%) along with maximum gain in callus biomass accumulation. This treatment also resulted in the lowest fresh-and dry-cell weight ratio (26.52) suggesting actual gain in callus biomass accumulation. Correlation analysis indicated a significant positive correlation between callus induction coefficient and cell fresh-(r = 0.939) and dry-(r = 0.951) weight.
Callus, secondary metabolites, plant growth regulators, Rosa hybrida
