1Division of Fruits and Horticultural Technology, ICAR-IARI, New Delhi
2Department of Plant Pathology, Bihar Agricultural College, Sabour, Bhagalpur
Division of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, New Delhi, 110 012
*Corresponding author's present address: Department of Horticulture (Vegetable and Floriculture), Bihar Agricultural College, Sabour, Bhagalpur, 813 210, E-mail: shyama.iari@gmail.com
Online published on 25 August, 2017.
An efficient protocol for in vitro multiplication of hybrid tea rose cv. Raktagandha was developed using axillary bud segments. Out of different pre-treatments of explants tried, the highest explant survival (78.05%) was obtained with carbendazim (0.2%) + diathane M-45 (0.2%) + 8-HQC (200 mg/l) for 3 h on a horizontal shaker (120 rpm). Sucrose concentration of 30 g/l in the medium seems to be optimal for in vitro shoot multiplication. Murashige and Skoog medium supplemented with 3.5 mg/l BAP + 0.2 mg/l NAA + 0.5 mg/l GA3 was found most effective for culture establishment and shoot proliferation with highest number of micro-shoots (4.62 shoots/explant). Rooting of micro-shoots was induced on half-strength MS basal medium supplemented with NAA (0.5 mg/l) + IBA (0.5 mg/l). The regenerated plantlets were efficiently hardened in glass jars filled with vermiculite + agropeat (1:2) moistened with half-strength MS medium salts and covered with polypropylene lids, thereafter plants were successfully transferred to the glasshouse with good survival.
Hybrid tea, micropropagation, protocol, rose
