Indian Journal of Horticulture
  • Year: 2019
  • Volume: 76
  • Issue: 2

Microsatellite maker based intervarietal variability study in Indian cauliflower

1Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India, 110012

Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi, 110012

*Corresponding author's E-mail address: hortparth@gmail.com

Online published on 12 July, 2019.

Abstract

The present study was conducted during 2015–2016 to assess molecular diversity in cauliflower. A total of 48 genotypes comprising early, mid-early and mid-late were characterized using 26 SSR markers. The polymorphic primers produced bands ranging from 2.00 to 5.00. A total of 76 alleles with an average of 2.24 alleles per locus were amplified in 48 cauliflower genotypes. Heterozygosity for individual loci among 48 genotypes ranged from 0.00 to 0.98. The UPGMA dendrogram allocated 48 cauliflower genotypes into six major clusters having maximum 8 genotypes (DB 15, CC 522, Selection 1–2, Maghi, PusaHimjyoti, AL 15, CC 35 and DC 431) in cluster I. The molecular markers employed in this study were effective in discriminating the 48 cauliflower genotypes.

Keywords

Brassica oleracea var. botrytis, variety, diversity, SSR