1Resident, Department of Pediatrics, SGT Medical College, Gurugram, Haryana, India
2Professor, Department of Pediatrics, SGT Medical College, Gurugram, Haryana, India
3Assistant professor, Department of Pediatrics, SGT Medical College, Gurugram, Haryana, India
4Professor, Department of Pediatrics, SGT Medical College, Gurugram, Haryana, India
5Emeritus Professor, Department of Microbiology, SGT Medical College, Gurugram, Haryana, India
Globally diarrheal diseases are the second leading cause of childhood mortality after pneumonia. Rotavirus is the most common identifiable viral cause of diarrhea in children below five years of age. Access to rapid and accurate diagnostic services for the detection of rotavirus in hospital is important not only for diagnosis of viral gastroenteritis, but also to prevent nosocomial spread of the disease and stop non-judicial use of antibiotics. The aim of the study was to compare rotavirus detection using Immunochromatography (ICG) and Enzyme linked immunosorbant assay (ELISA) in children with acute gastroenteritis. The study was carried out in the department of Microbiology, SGT Medical College on 85 children of age less than two years who presented with symptoms of acute gastroenteritis over a period of eight months. Stool sample was collected in wide mouth container. VP6 antigen detection was done by Immunochromatography (ICG) and Enzyme linked immunosorbent assay (ELISA) as per manufacturers guidelines. Of 85 stool samples tested 14 samples by ICT and 11 samples by ELISA were positive for rotavirus. ICG shows good agreement with ELISA and has the advantage of being quicker, cost effective and convenient to perform with easy to read results.
Group A Rotavirus, ELISA, ICG, Comparison, Acute gastroenteritis
