Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India
*Corresponding author; E-mail: tsnarayana@vsnl.net Tel: 91(11)24102008; Fax: 91(11)26885270
A thermophilic mould Thermomucor indicae-seudaticae was shown to be amenable for the production of a thermostable and neutral glucoamylase in solid-state fermentation. The mould produced the enzyme optimally at 40 ºC and pH 7, when grown in wheat bran moistened with a salt solution in 1:2.5 (w/v) ratio. A very high enzyme titre (392.3±5.5 U g−1 DMB) was attained when wheat bran was supplemented with 2% cotton oil seed cake.
Solid state fermentation, thermophilic mould, starch, glucoamylase, Thermomucor indicae-seudaticae
