1Molecular Genetics and Microbiology, The University of Texas, University Station A5000, Austin TX 78712-0162, USA
2Department of Biotechnology, Punjab University, Chandigarh 160 014, India
3Department of Microbiology, Punjab University, Chandigarh 160 014, India
*Corresponding author; E-mail: ineetudahiya@yahoo.com. Tel: 91(172)2541770; Fax: 91(172)2541409.
Bacillus megaterium AKG-1 produced lipase during solid substrate fermentation. Maximum lipase yield was 18.6 IU g−1 (wet wt) with wheat bran as solid substrate. Lipase yield was 42% more when wheat bran-soybean mixture was used. Supplementation of medium with Tween-80 (0.5%) led to 76% increase in lipase production. Addition of soybean oil (1-2%) to wheat bran had no effect on lipase yield.
Lipase, Bacillus megaterium AKG-1, solid substrate fermentation, wheat bran, submerged fermentation
