Biotechnology Division, Defence Research & Development Establishment, Jhansi Road, Gwalior, Madhya Pradesh 474 002, India
*Corresponding author; E-mail: lst2397@rediffmail.com Tel: 91(751)22340245, 2340345, Fax: 91(751)2341148
Anaerobic consortium for degradation of night soil was subjected to five cycles of freeze/thaw treatment. The performance of inoculum sharply declined after first cycle of treatment with gradual decrease after subsequent cycles. The deleterious effect of freezing was most pronounced for methanogens and sulfate reducing bacteria.
Anaerobic consortium, freezing injury, night soil
