Environmental Physiology and Biotechnology G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora 263 643, Uttaranchal, India
1State Biotechnology Programme, Govt. of Uttaranchal, Biotech Bhavan, P.O. Haldi, U.S. Nagar, 263 146.
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Saccharomycopsis fibuligera, a mycelial yeast, was isolated from the soil samples collected from Soldhar, a hot spring located in the Garhwal Himalayas and investigated for morphological, biochemical and physiological characteristics. The yeast was found to produce extracellular amylase on soluble starch, having 73 units mL−1 activity and temperature and pH tolerance from 4 to 60°C (optimum 40°C) and 4–11 (optimum 6 pH). The Km and Vmax values at 37°C were 1.1 mg mL−1 and 0.0131 μmoles min−1 mL−1. The PAGE analysis showed a band for the crude enzyme with a molecular weight corresponding to 66 kDa.
Saccharomycopsis fibuligera, amylase, temperature, pH
