Indian Journal of Microbiology

  • Year: 2005
  • Volume: 45
  • Issue: 3

Extracellular amylase activity of Saccharomycopsis fibuligera, a mycelial yeast isolated from a hot spring site in Garhwal Himalayas

  • Author:
  • Bhavesh Kumar1, Anita Pandey1, Lok Man S. Palni2
  • Total Page Count: 5
  • DOI:
  • Page Number: 211 to 215

Environmental Physiology and Biotechnology G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora 263 643, Uttaranchal, India

1State Biotechnology Programme, Govt. of Uttaranchal, Biotech Bhavan, P.O. Haldi, U.S. Nagar, 263 146.

Abstract

Saccharomycopsis fibuligera, a mycelial yeast, was isolated from the soil samples collected from Soldhar, a hot spring located in the Garhwal Himalayas and investigated for morphological, biochemical and physiological characteristics. The yeast was found to produce extracellular amylase on soluble starch, having 73 units mL−1 activity and temperature and pH tolerance from 4 to 60°C (optimum 40°C) and 4–11 (optimum 6 pH). The Km and Vmax values at 37°C were 1.1 mg mL−1 and 0.0131 μmoles min−1 mL−1. The PAGE analysis showed a band for the crude enzyme with a molecular weight corresponding to 66 kDa.

Keywords

Saccharomycopsis fibuligera, amylase, temperature, pH