Department of Studies in Applied Botany and Seed Pathology, University of Mysore, Manasagangothri, Mysore-570 006, Karnataka, India
*Corresponding author; E-mail: hsp@appbot.uni-mysore.ac.in Tel/Fax: 91 821 2414450
Bean common mosaic potyvirus (BCMV) is an important seedborne pathogen of french bean. BCMV was purified to homogeneity employing an improved purification protocol using ammonium sulphate precipitation instead of polyethylene glycol precipitation step. Polyclonal antibody was produced in rabbit for purified virus fraction and characterized for its antigenic determinant by western blotting and the results were compared with MAb-BCMV. PAb-BCMV i) had an antibody titer of 1: 3000; ii) sensitive as it could detect 50 to 250 μg of total protein in crude sap and 10 to 50 μg of purified virus fraction; iii) specific, as it detected only BCMV and not other potyviruses except peanut mottle virus which is not a french bean pathogen; and iv) relevant in seed health testing as it exhibited broad-spectrum reactivity with all isolates and found to be suitable for seed health testing of french bean.
Bean common mosaic virus, purification, polyclonal antibody, antibody titer, health testing
