1Ph.D Scholar, LNCT UniversityKolar Marg, Sarvadharam C-Sector, Shirdipuram, Sarvadharam, Bhopal, Madhya Pradesh, 462042
2Professor and Head of Department of Microbiology, LNCT UniversityKolar Marg, Sarvadharam C-Sector, Shirdipuram, Sarvadharam, Bhopal, Madhya Pradesh-462042
Online Published on 31 August, 2023.
Antifungal susceptibility testing is a subject of interest in the field of medical mycology which deals with effectiveness of antifungal drugs. The aim of the present study was the identification, distributions and antifungal susceptibility patterns of various Candida Krusei isolated from various clinical specimens in the multispecialty hospital.
A clinical samples will be submitted to Dept. of Microbiology L.N. Medical College Bhopal for routine diagnostic workups were assessed. of 310 Candida samples 41 samples have Candida Krusei so 41 sample that have Candida krusei were used for further study for antifungal susceptibility testing by a battery of microbiological investigations aimed at detecting, isolating, identifying and characterizing the Candida sp. so as to determine the spectrum of Candidiasis.Samples were processed by Gram staining, KOH mount and culture on SDA and BHI agar. Isolated yeasts were identified and speciated by germ tube test, chlamydospores formation on corn meal agar, color production on CHROM agar, sugar fermentation test and sugar assimilation test. Antifungal susceptibility testing of isolates was performed as per CLSI guidelines.
Out of 310 samples of candida contains 41 (13.00%) samples contain Candida Krusei. In our study out of total candida species in 310 samples.Candida albicans more prevalent rather than other candida species in various clinical samples.Out of total 41 samples of Candida Krusei 41 (100.00%) samples were found susceptible for Amphotericin-B and 0 (0.00%) samples were resistant for Amphotericin –B. Fluconazole were shows 35 samples that was 85.37% of total samples were found susceptible and 6 samples were resistant that was make 14.63% of total samples. Variconazole was show susceptibility for 38 samples that was making 92.68% of total 61 samples and resistant for 3 samples that was form 7.32% of total samples. Itraconazole was show susceptibility for 38 samples that was making 92.68% of total 61 samples and resistant for 3 samples that was form 7.32% of total samples. . Nystatin was show susceptibility for 38 samples that was making 92.68% of total 61 samples and resistant for 3 samples that was form 7.32% of total samples.
The present study reported a significant shift in incidence of invasive Candidiasis from C.albicans to Non albicans species. Non -albicans Candida has emerged as an important opportunistic pathogen. Non-albicans species are assuming an increasing role in nosocomial infections particularly in infants. The incidence of Candidaemia caused by non-albicans species is frequent and increasing significantly.
Candida Species, Nosocomial, Antifungal Resistance, Infants
