Lecturer, Department of Bio Technology, Apex Group of Institution, INDRI Road, KARNAL
Online published on 26 June, 2013.
Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. However, somatic cloning has been inefficient in all species in which live clones have been produced. High abortion and fetal mortality rates are commonly observed. These developmental defects have been attributed to incomplete reprogramming of the somatic nuclei by the cloning process. Various strategies have been used to improve the efficiency of nuclear transfer, however, significant breakthroughs are yet to happen. This paper include studies conducted and to gain a better understanding of nuclear reprogramming. Because cattle are a species widely used for nuclear transfer studies, and more laboratories have succeeded in cloning cattle than any other specie.
Molecular cloning, organism cloning (artificial cloning of organism), creating recombinant DNA