1Assistant Professor, Department of Microbiology, Shimoga Institute of Medical Sciences, Rajiv Gandhi University of Health Sciences, Shivamogga, Karnataka, India
2Associate Professor, Department of Internal Medicine, Shimoga Institute of Medical Sciences, Rajiv Gandhi University of Health Sciences, Shivamogga, Karnataka, India
3Professor and Head, Department of Microbiology, Shimoga Institute of Medical Sciences, Rajiv Gandhi University of Health Sciences, Shivamogga, Karnataka, India
4Associate Professor, Department of Microbiology, Shimoga Institute of Medical Sciences, Rajiv Gandhi University of Health Sciences, Shivamogga, Karnataka, India
The increasing prevalence of Methicillin resistant among staphylococci (MRSA) is an increasing problem. Increasing incidence of infections due to MRSA has led to emphasis on the need for safe & effective agents to treat both systemic & localized Staphylococcal infections. Clindamycin has been used to treat pneumonia & soft tissue and musculoskeletal infections due to MRSA. One important issue in Clindamycin treatment is the risk of clinical failure during therapy caused by MLSB inducible resistance.
To isolate and identify Staphylococcus aureus and CONS from all clinical samples & to determine the inducible Clindamycin resistance among the Staphylococcus aureus and CONS.
A total of 100 isolates of Staphylococcus aureus and CONS from various samples were isolated. Methicillin resistance was detected by using a 1 μg Oxacillin disc. The D-test was performed using the discs of Clindamycin (CL)(2μg) and Erythromycin (ER)(15μg) placed at a distance of 15mm (centre to centre) along with routine antibiotic susceptibility testing.
Among Staphylococcus aureus, MSSA isolates were 32(47.05%) compared to MRSA isolates, 26(38.24%) and among CNS, MSCONS isolates were 8(11.77%) compared to MRCONS 2(2.9%).A total of 12(17.64%) isolates showed iMLSB, of which 8(11.77%) were MRSA, 2(2.9%) were MSSA and 2(2.9%) MRCONS isolates.
Prevalence of inducible Clindamycin resistance among Staphylococcal isolates was significant. Hence the implementation of this D-test routinely, which is simple, reliable & inexpensive will reveals the iMLSB & cMLSB phenotype & prevents the therapeutic failure of Clindamycin.
Methicillin resistant Staphylococcus aureus (MRSA), Inducible Clindamycin resistance, D-test, Erythromycin, iMLSB phenotype
