Indian Journal of Microbiology Research

  • Year: 2016
  • Volume: 3
  • Issue: 4

Comparative study of Virulence factors among MBL and Non MBL producing Urinary isolates of Pseudomonas aeruginosa in a tertiary care hospital

1Associate Professor, Dept. of Microbiology, Mahatma Gandhi Medical College & Research Institute, Puducherry, Sree Balaji Vidyapeeth University

2Professor, Dept. of Microbiology, Mahatma Gandhi Medical College & Research Institute, Puducherry, Sree Balaji Vidyapeeth University

3Professor & Head, Dept. of Microbiology, Mahatma Gandhi Medical College & Research Institute, Puducherry, Sree Balaji Vidyapeeth University

Abstract

The study was done to detect various virulence factors of urinary isolates of Pseudomonas aeruginosa with special reference to MBL producing strains and to find out its significant association with the virulence factors.

This study was done in a tertiary care teaching hospital in Pondicherry, which included 50 urinary isolates of Pseudomonas aeruginosa. All isolates subjected to routine antibiotic susceptibility testing by Kirby Bauer Disc Diffusion method and screened for Metallo β-lactamase (MBL) by Imipenem-EDTA disc method. Phenotypic detection of virulence factors like phospholipase, hemolysin, gelatinase and DNAse were done for the same.

Out of 50 isolates of Pseudomonas aeruginosa, 26% were MBL producers and 74% were non MBL producers by Imipenem-EDTA disc method. Virulence factors like hemolysin, gelatinase, phospholipase and DNAse were shown in 88%, 78%, 76% and 50% respectively. The association with the production of Virulence factors and MBL production were found to be statistically significant only in case of DNAse production.

This study concluded that P. aeruginosa is a pathogen able to accumulate various virulence factors which are often accompanied by multidrug resistance and pan-resistance, making the treatment of infections difficult for the clinicians.

Keywords

DNAse, Gelatinase, Hemolysin, Mettalo β-lactamase, Phospholipase