Ethanol production from sugarcane bagasse hydrolysate using newly adapted strains of Meyerozyma guilliermondi CBA-524 and Pichia kudriavzevii CBA-519

New strains of the yeasts Meyerozyma guilliermondi CBA-524 and Pichia kudriavzevii CBA-519, capable of growing on sugarcane bagasse hydrolysate, have a great potential for second-generation bioethanol processes. Meyerozyma guilliermondi CBA-524, achieved a maximum ethanol concentration of 3.01g/L corresponding to a yield of 0.26 g ethanol per g of sugar and a productivity of 0.125 g/l/h, after 24 h of cultivation. Pichia kudriavzevii CBA-519 exhibited a prolonged fermentation time on bagasse hydrolysate resulting in an ethanol yield of 0.26 g/g and a productivity of 0.015 g/l/h after 192 h of fermentation. The ethanol yield and the volumetric productivity using the adapted P. stipitis CBS6054 standard were 0.34 g/g and 0.083 g/l/h, respectively, after 48 h of cultivation. Furthermore, the newly adapted M. guilliermondi CBA-524 demonstrated significantly enhanced tolerance to sugarcane bagasse hydrolysate, exhibiting a maximum in ethanol concentration after 24 h of fermentation. More adapted yeasts and improved fermentation conditions for this yeast can be of great importance for the increased production of ethanol using sugarcane bagasse hydrolysate.