Indian Journal of Microbiology Research
  • Year: 2017
  • Volume: 4
  • Issue: 2

DNA fingerprinting and drug resistance profiles of Mycobacterium tuberculosis strains isolated from HIV-infected pulmonary tuberculosis patients

  • Author:
  • Santosh S. Patil1,, T Mohite Shivajirao2, Subarna Roy3, Shuchismita Dey4
  • Total Page Count: 6
  • Page Number: 210 to 215

1Associate Professor, Bharati Vidyapeeth Deemed University, Medical College, Sangli, Maharashtra

2Krishna Institute of Medical Sciences, Deemed University, Karad

3Scientist E, National Institute of Traditional Medicine, Belgavi, Karnataka

4Research Fellow, National Institute of Traditional Medicine, Belgavi, Karnataka

*Corresponding Author: Email: drsantoshpatil21@gmail.com

Online published on 3 April, 2018.

Abstract

Tuberculosis (TB) is a leading cause of morbidity and mortality among HIV/AIDS patients. These patients are more likely to develop resistance to anti-tuberculosis drugs as well. Identifying open cases of TB, studying their drug susceptibility pattern and knowledge of genetic diversity to know transmission dynamics among HIV-TB patients is therefore important to control TB.

1) To determine the prevalence of HIV, among sputum positive pulmonary tuberculosis (PTB) patients. 2) To study drug resistance pattern of Mycobacterium tuberculosis isolates. 3) To evaluate genotypic diversity of M. tuberculosis isolates using random amplified polymorphic DNA (RAPD) technique.

Hospital based cross sectional study

M. tuberculosis strains were isolated from HIV seropositive PTB patients with irrespective of their anti-TB treatment. These isolates were processed for drug sensitivity testing (DST) by Proportion method and subsequently genotyped by RAPD technique.

Prevalence of HIV-TB co-infection was 22.2%. Total 52.1% M. tuberculosis isolates were resistant to one or more number of drugs. We report 17.4% MDR-TB among study population. RAPD-PCR showed excellent discrimination with high degree of polymorphism among M. tuberculosis isolates. Transmission and diversity of tuberculosis was 10% and 90% respectively.

17.4% of MDR-TB among studied patients indicated worrisome trend that calls for continuous monitoring and DST. RAPD analysis is quick, simple and has potential for molecular epidemiology of TB. RAPD-PCR showed notable genetic polymorphism, suggesting that source and type of infections within the population were varied and no any particular clone of M. tuberculosis is spreading among study population.

Keywords

HIV-TB, RAPD-PCR, Pulmonary tuberculosis, DNA fingerprinting