Ex vivo study of garcinia mangostana L. (Mangosteen) peel extract and xanthones as anti-adipogenesis in HepG2 cell model

Anti-adipogenesis is one of proposed mechanism for anti-obesity. Adipogenesis regulation of obesity is important, so identification of anti-adipogenic activity is a potential strategy to find anti-obesity agent.

The aim of this study is to evaluate the anti-adipogenesis potential of Garcinia mangostana L. peel extract (GMPE) compared to xanthones in HepG2 cells line as model.

GMPE was performed based on maceration method using distilated ethanol 70% as the solvent. The level of triglyceride and cholesterol and the inhibitory activity of triglyceride (TG) and cholesterol (CHOL) in HepG2 cells were assayed and determined as the anti-adipogenesis parameter.

The most active subtance to lower the triglyceride level was showed by GMPE in every concentration followed by the garcinone-C, y-mangostin, garcinone-D and amangostin respectivelly. The highest activity to decrease the cholesterol level was showed by GMPE and followed by y-mangostin, a-mangostin, garcinone-c, garcinone-d respectively.

GMPE posses the anti-adipogenesis potential in inhibiting TG and CHOL synthesis was better than any other xanthone (amangostin, y-mangostin, garcinone-C and garcinone-D).