Evaluation tissue dissolution property of 2.5% sodium hypochlorite prepared by hydrochloric acid and sodium bicarbonate: An in vitro

Successful endodontic treatment requires chemical preparation in addition to mechanical preparation. The most common material for chemical preparations is sodium hypochlorite. One way to reduce the effects of pH adjustment is the use of sodium hypochlorite. The present paper was conducted to examine the effect of dilution with hydrochloric acid and sodium bicarbonate and reduce pH on ability of tissue solubility of sodium hypochlorite. The present study was conducted in vitro on bovine muscle tissue. Ability of tissue solubility was conducted in four groups respectively with active ingredient including 1) sodium hypochlorite diluted with distilled water 2) sodium hypochlorite diluted with sodium bicarbonate 3) sodium hypochlorite diluted with hydrochloric acid and finally 4) distilled water (control group). Each sample was firstly weighed and then placed in contact with 10 m/L solution for 60 minutes (five 12 -minute intervals). The sample was weighted every five minutes and solution was renewed. The results were analyzed using SPSS-21 Software based on variance analysis, Tukey and T-test (α=0.05). The findings showed that there was significant difference between first, second and third groups in terms of ability of tissue solubility. However, the tissue solubility in second and third groups was lower than first group and it was similar in second and third groups (P Value <0.001). Reduction of sodium bicarbonate PH using sodium hypochlorite and hydrochloric acid reduces ability of tissue solubility in sodium hypochlorite.