Key Laboratory of Biochemistry and Molecular Biology, The Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing, China
*Corresponding e-mail: mayongping@cqmu.edu.cn
Online published on 28 February, 2019.
For a conventional PCR, the total reaction times of a certain DNA amplification using conventional PCR were minimized to more than a half. The extension time could be calculated as the formula: y=0.005x-5.2679 (y represented the extension time (sec) and x represented the DNA length (bp); y ≥ 1.0). The annealing time was minimized as short as 1 sec. However, the Ta was changed from Tm ± 6.0°C.
PCR, Rapid, Parameter optimize Highlights
