1Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India
2Amity Institute of Molecular Medicine and Stem Cell Research, Amity University, Noida, Uttar Pradesh, India
3Department of Medical Oncology, IRCH, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India
4Department of Pulmonary Medicine and Sleep Disorders, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India
5Department of Research, Sir Ganga Ram Hospital, Rajinder Nagar, New Delhi, India
*Corresponding e-mail: sbose1@amity.edu
Online published on 1 October, 2020.
Obstructive sleep apnea (OSA) is a respiratory disorder with multiple clinical outcomes and is now being recognized as a serious health concern across the globe. However, the mechanisms of its pathophysiology are still elusive. Also, to date, evidence of miRNA regulation of sleep apnea in the Indian sub-population is unknown.
In this study, we investigated the expression pattern of certain potential obesity-linked miRNAs in OSA subjects. Seventy adult subjects (20 obese OSA, 20 non-obese OSA and 30 healthy) were selected for this study. Total RNA was extracted and the expression of miR-21, miR-27, miR-29 and let-7 (normalized with internal control RNU48) was analyzed by SYBR Green-based qPCR.
We selected miR-21, miR-27, miR-29 and let-7 for their-documented role in obesity. Relative miRNA expression profiles revealed differential expressions of all four above-mentioned miRNAs in both obese and non-obese OSA subjects compared to healthy controls. Statistical analysis-revealed a significant correlation between miRNA expression with obesity-associated parameters in OSA subjects.
Our study demonstrates the involvement of four miRNAs (miR-21, miR-27, miR-29 and let-7) as potential-molecular players of obesity-associated OSA. Identification of miRNA targets and in-depth analysis of their molecular-mechanism in disease pathogenesis is further warranted.
Obstructive sleep apnea, Microrna, Obesity, Differential expression
