Biodegradation of toxic monomer acrylamide using microorganism

Acrylamide can be produced at high level in high carbohydrate heat treated foods. It is a well-known neurotoxicant, Carcinogen and teratogen. In the present Investigation, soil samples were collected and enriched with potato chips and 100 PPM acrylamide for 15 days. The enriched samples were serially diluted, and spread plated on nutrient agar medium, amended with 100 PPM acrylamide. Three dominant isolates of bacteria were obtained. Minimum inhibitory concentrations of the selected isolates were carried out against increasing concentration of acrylamide, ranging from 200 PPM to 900 PPM. All the three isolates (AC1, AC2 & AC 3) exhibited tolerance up to 600 PPM. The strain AC1 displayed maximum tolerance of acrylamide up to 900 PPM. On the basis of the AC1 was selected for further characterization and degradation studies. The effective acrylamide tolerant isolate was identified as Bacillus subtilis. Biodegradation of acrylamide was performed at various concentrations 200 PPM and 500 PPM. To study of FTIR and HPLC analysis was also performed with 500 PPM. Bacillus subtilis grew optimally at pH 7 and temperature 30 and 35°C. The carbon and nitrogen sources were tested. Acrylamide degrading strains were analyzed plasmid DNA by Alkaline Lysis method.