Eight Pathogenicity experiments, four on M. incognita and four on R. reniformis were conducted. These studies revealed that 10 days old seedlings of grape (Anab-E-Shahi) were killed by M. incognita within 30 days of inoculation with an initial population level of 1000 nematodes per pot (500 g soil) and above, while 1,00,000 larvae of Rotylenchulus reniformis were able to kill only 3 plants out of 7. No death was observed in seedlings of 60 days and above and also in cuttings of 4 months age. Inoculations on 60 days old seedlings at 90 days after inoculation showed more damage by 1000 level of nematodes than 10,000 level in both the nematodes.
