Free aminoacids changes in the roots of blackgram infected with root-knot nematode, Meloidogyne incognita

Chromatographic analysis for free aminoacids of blackgram (var. T-9) roots infected with root-knot nematode, Meloidogyne incognita under pot culture conditions revealed the presence of 9 free amino acids such as L-aspartic acid, L-glutamic acid, L-asparagine, L-serine, glycine, L-threonine, L-tryptophan, L-phenylalanine and iso-leucine in healthy root extract, whereas in nematode infected root extract, L-glutamic acid was absent but 4 more aminoacids namely L-cystine, L-lysine, L-proline and L-leucine were present. There wall no apparent differences between 15 and 30 days samples with regard to the number of aminoacids present except their higher concentration in later samples particularly in infected tissue.