Division of Nematology, Indian Agricultural Research Institute, New Delhi.
Cysts of Heterodera avenae extracted through zinc sulphate, magnesium sulphate, acetone and ethanol + glycerine have been compared with manually extracted ones with regard to efficiency of the method and their effect on larval penetration and development inside wheat roots. Manually picked cysts were superior in all aspects. Amongst the chemicals zinc sulphate and magnesium sulphate, were least harmful. Schemes for extraction and separation of cysts of H. avenae from soil are provided.
Extraction techniques, biology, Heterodera avenae