Laboratory experiments were conducted to evolve a suitable method for the storage of Heterodera avenae cysts, which would provide the encysted eggs without loss of viability and infectivity for use in investigations by the researchers. Cysts were stored for six months in four media (filter paper, dry sandy soil, mineral oil and agar gel) and kept at two temperatures (30°C and 7–10°C). Viability of the encysted eggs has been judged by the emergence of juveniles and infectivity by the penetration and multiplication of the nematode in/on wheat roots. The results revealed that the encysted eggs of H. avenae could be conveniently stored for atleast six months in dry sandy soil at 30°C without losing the viability and infectivity.
Storage method, Heterodera avenae cysts
