1Division of Nematology, Indian Agricultural Research Institute, New Delhi-110 012.
2Central Potato Research Institute, Ootacamund, Tamil Nadu.
Random amplified polymorphic DNA (RAPD) markers are very efficient tools for detecting intra-specific genetic variability. A random operon primer OPG 5 that can differentiate between the two species of Globodera has been used to characterize the gene pool similarity of the field populations of potato cyst nematode from Nilgiri Hills. Of 44 individual cysts analyzed by RAPD-PCR, 41 had same RAPD profile and they were assigned to G. rosrochiensis. However, 3 individuals were different which could be variants of G. rostochinsis.
Potato cyst nematode, DNA/molecular markers, RAPDs, genetic variability, resistance breeding, quarantine
