Differential induction of chitinase activity in resistant and susceptible sugarcane clones infected by Pratylenchus zeae
Abstract
The chitinase activity in roots and leaves of sugarcane clones in response to P. zeae infection was compared in four resistant (BO 99, Co 86010, Co 86011 and CP 52 - 1) and two susceptible (CoC 85061 and CP 44 - 101) clones at 14 days after infection. In both roots and leaves, the highest chitinase activity was recorded when compared to control in resistant clone Co 86011 and lowest in CoC 85061. In western blot analysis, totally nine isoforms of chitinase were recorded. The isoform 25 kDa was found only in nematode infected resistant clones (Co 86011 and BO 99). The isoform 55 kDa was eliminated in nematode infected susceptible clone (CP 44–101).
Keywords
Chitinase, enzyme assay, lesion nematode resistant, sugarcane, western blot
