1Entomology Division, Central Rice Research Institute, Cuttack, Orissa-753 006, India
2Nematology Department, Orissa University of Agriculture and Technology, Bhubaneswar-751 003, India
Three rice varieties, Annapurna (susceptible), Manika (moderately resistant) and Ramakrishna (resistant), were grown in pots in a greenhouse and inoculated with rice root-knot nematode (Meloidogyne graminicola) @ 1 J2/g soil. Infected plant roots of Annapurna, Manika and Ramakrishna produced greater amount of phenolics, by 27.7, 47.6 and 104.2% respectively as compared to their healthy ones. Similarly activity of enzyme, Phenylalanine ammonia lyase (PAL) increased by 16, 50.26 and 63.24% correspondingly, reducing amount of the aminoacid phenyl alanine content by 16.39, 35.4 and 68%. Tyrosine ammonia lyase (TAL) activity increased in the infected root by 9, 19.18 and 53.89% with 1.89, 18.6 and 35.89% decrease in the contents of amino acid tyrosine. Amino acid tryptophan decreased @ 27.65, 23.07 and 13.63% respectively. Reaction of the varieties to the nematode can be ascertained by analyzing these biochemicals.
Rice, Meloidogyne graminicola, phenolics, phenyalanine ammonia lyase and tyrosine ammonia lyase, resistance
