Random Amplified Polymorphic DNA Markers for Prediction of Resistance in Tomato against Root-Knot Nematode, Meloidogyne incognita

The random amplified polymorphic DNA (RAPD) technique has been used for species and cultivar polymorphism detection and identification. Molecular characterization of eight tomato varieties/lines (Pusa Ruby, SL 120, Pusa Sheetal, Hisar Lalit, Aruna, N1 and N2) was done with the help of random primers and polymorphism studied among resistant and susceptible varieties. Thirty five operon primers (OPA, OPB and OPE series) were screened in the study. Only 3 primers OPA 1, OPA 3, OPA 13 exhibited 36.3, 30 and 44.4% polymorphism, respectively, single unique band showing variation among resistant and susceptible varieties was obtained in case of OPA 1 and OPA 3 but was absent with OPA 13.