Root-Knot Nematode, Meloidogyne incognita and Salicyclic Acid Induce Production of PR-Proteins in Tomato
Abstract
Foliar spray with Salicylic acid (100 μ/ml) or Meloidogyne incognita (2 J2/g soil) infection on tomato induced activity of chitinase and β-1,3-glucanase proteins. The levels of these proteins peaked on the 3rd day after treatment. The SDS-PAGE protein profile of the concentrated apoplastic fluid from the Salicylic acid treated and root-knot nematode inoculated showed presence of 35 kD and 26 kD proteins only in the SA spray and root-knot nematode inoculated plants. These proteins cross reacted in the preliminary immunoblotting with antiserum raised against pathogenesis related proteins, β-l,3-glucanase and a chitinase from tobacco respectively.
Keywords
Tomato, Meloidogyne incognita, pathogenesis related (PR) proteins, chitinase, β-l,3-glucanase, salicylic acid
