1Department of Bio-Sciences, H.P. University, Shimla-171 005, India.
2Department of Nematology, CCS Haryana Agricultural University, Hisar-125 004, India.
Two new species of Aphelenchoides and one of Laimaphelenchus from dead twigs of Pinus roxburghii Sarg. from Himachal Pradesh, India are described and illustrated. A. depressospicularis sp. n.: Female: L = 0.60–0.75 mm; a = 32–45; b = 9.5–11.5; b' = 4.3–5.2; c = 14–19; c' = 2.0–3.4; V = 63–72; stylet = 8–11 ~m; Male: L = 0.50–0.60 mm; a = 31–39; b = 8.4–10.3; b' = 4.0–5.1; c = 15–22; stylet = 8–11 ~m; stylet with knobs; dorsal limb of spicule 16–19 ~m long with a depression near tip. A. microspermi sp. n.: Female: L = 0.42–0.66 mm; a = 31–46; b = 7.4–10.1; b' = 3.3–4.6; c = 13–19; c' = 3.2–4.6; V = 69–75; stylet = 8–11 ~m, post vulval uterine sac filled with numerous sperm. Male: L = 0.40–0.59 mm; a = 28–40; b = 7.1–10.1; b' = 3.5–4.4; c = 16–20; stylet =: 8–10 ~m; spicule = 13–15 ~m; sperm small sized. Laimaphelenchus simlaensis sp. n.: Female: I-, = 0.84–1.09 mm; a = 38–46; b = 11–14; b' = 4.6–6.2; c = 21–30; c' = 2.3–3.6; V = 67–73; stylet = 11–13 ~m; vagina without sclerotization; tail provided with a single stalked, sucker-like tubercle bearing 3–5 fine processes. Male: L = 0.78–1.02 mm; a = 41–48; b = 11–14; b' = 4.2–5.3; c = 23–29; c' = 2.0–2.3; stylet = 11–13 ~m; spicule = 16–18 ~m, gubernaculum-like structure present.
Aphelenchoides depressospicularis sp. n, A. microspermi sp. n, Laimaphelenchus simlaensis sp. n, Pinus roxburghii, dead twigs