Effect of Culture Filtrates of Trichoderma viridae and Trichothecium roseum Grown on Different Carbon and Nitrogen Sources on Hatching and Mortality of Root-Knot Nematode (Meloidogyne incognita)

An investigation was undertaken to study the effect of different carbon and nitrogen sources on the growth and antagonistic properties of Trichoderma viridae and Trichothecium roseum. Mycelial growth and phenols in the culture filtrates of fungi were estimated. Cumulative hatching and percentage mortality of root-knot nematode (Meloidogyne incognita) juveniles were observed at 2, 3, 5 and 7 days. Results indicated varying effect of nutritive sources on the mycelial growth, glucose and Potassium Nitrate were a most preferred while starch and urea were least preferred carbon and nitrogen sources. Hatching and mortality also varied with the different carbon and nitrogen sources. Though all sources proved inhibitory, most effective were fructose and lysine in case of T. viridae and rhamnose and glycine for T. roseum. It was observed that a direct correlation between the amount of phenols in the culture filtrate of fungi and its adverse effect on the hatching and mortality of juvenile of root-knot nematode.