In silico Analysis of Root-Knot Nematode, Meloidogyne incognita House-Keeping Gene

The nematode house-keeping genes play a key role in basic functions of nematode metabolism and development. Although substantial research on house-keeping genes has been carried out in Caenorhabditis elegans, but their genomics and transcriptomics studies in plant-parasitic nematodes (PPNs) are limited. In the present investigation, a house-keeping Integrase gene from the southern root-knot nematode, Meloidogyne incognita was characterized through bioinformatics tools. A partial segment of 651 bp was analysed on Gbrowse to obtain 1171 bp long full CDS specifying the open reading frame. The deduced amino acid sequence showed 100% similarity with Minc01176 gene from M. incognita. The domain prediction analysis revealed that two SNFC-5 pfam domains are present in the predicted protein which has functions in chromatin remodeling complex. A phylogenetic tree based on the amino acid sequences showed evolutionary relationship of Minc01176 gene with house-keeping genes of other nematodes. The 3D models and Ramachandran plot for Minc01176 gene was predicted with the best confidence score at-1.73.