International Journal of Nanotechnology and Applications
  • Year: 2009
  • Volume: 3
  • Issue: 1

Protective Effects of Nano-ZnO on the Primary Culture Mice Intestinal Epithelial Cells in in vitro Against Oxidative Injury

  • Author:
  • AI Dawei1,2, WANG Zhisheng1,2,, ZHOU Anguo1,2
  • Total Page Count: 6
  • Page Number: 1 to 6

1Animal Nutrition Institute, Sichuan Agricultural University, Ya'an, 625014, China.

2Engineering Research Center of Animal Disease-Resistance Nutrition of China, Ministry of Education, Sichuan Agricultural University, Ya'an, 625014, China.

*Corresponding author: Professor WANG Zhisheng, Institute of Animal Nutrition, Sichuan Agricultural University, 625014, Ya'an, Sichuan, China.

Abstract

Nano-ZnO is a new products which may used to be feed resources. The aim of this study was to exam whether nano-ZnO is able to protect cell integrity from potential damage by free-radical oxidative injury. A 3×3 factorial experiment was used to investigate the interaction between particle diameter (15, 50 and 100nm) and concentration (0.4, 1.6 and 6.4 μg mL−1) of the primary culture mice intestinal epithelium cell (IEC) in in vitro. The results indicated that the all the nano-ZnO groups MTT OD value were significantly higher than the perhydrol group (P <0.05), and LDH decreased, but not significant (P>0.05). The MDA content in all the nano-ZnO groups were decreased significant (P<0.01). The SOD and CAT in all nano-ZnO groups were higher than the perhydrol group (P>0.05). The results showed that nano-ZnO released the IEC oxidative injury.

Keywords

Intestinal epithelium cell, nano-ZnO, antioxidant, antioxidant enzymes, integrity