1Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, 68131, U.S.A.
2Sardar Vallabh Bhai Patel University of Agriculture & Technology University, Meerut, 250110, (U.P.), India.
3Department of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture and Technology, Pantnagar, India.
RNA interference (RNAi) is an evolutionarily conserved mechanism that causes sequence specific gene silencing, when triggered by dsRNA. The ever-growing list of potential applications has contributed to the rapid growth of this technique and to the understanding of its biology. Though biologically evolved to mediate resistance to both endogenous and exogenous pathogenic nucleic acids and to regulate the expression of protein coding genes, it is being successfully used as a tool for functional genomics and to manipulate the expression of desired genes.
