Indian Journal of Crop Science
  • Year: 2006
  • Volume: 1
  • Issue: 1–2

Amino acids enhancing plant regeneration from cotyledon and embryonal axis of peanut (Arachis hypogaea L.)

  • Author:
  • K. Vasanth1,, A. Lakshmiprabha2, N. Jayabalan2
  • Total Page Count: 5
  • Page Number: 79 to 83

1Institute of Wine Biotechnology, Department of Viticulture and Oenology, Stellenbosch University, South Africa-7602

2Plant Biotechnology, School of Life Sciences, Bharathidasan University, India-620 024

*Present address: Plant Biotechnology, School of Life Sciences, Bharathidasan University, India-620 024

Abstract

The effects of amino acids (glutamine, serine, proline, glycine and valine) and additives (PVP, sodium citrate, activated charcoal) on plant regeneration from cotyledon and embryonal axes explants of peanut were studied. Cotyledon showed better response than embryonal axis explants. The combination of BA (1.5mM), NAA (1.0mM), PVP (25mM) with L-glutamine (15mM) enhanced the maximum number of shoots (36.2 shoots/explants). The multiple shoots were separated and transferred to shoot elongation medium containing BA (1.0 mM) and GA3 (0.5mM). The elongated shoots were rooted on MS medium containing NAA (1.0mM). A high percentage of (97.5%) plants were successfully hardened.

Keywords

Cotyledon, Embryonal axes, Amino acids, BA-benzyl amino purine NAA-a-Naphthaline acetic acid