Indian Journal of Oral Health
  • Year: 2012
  • Volume: 2
  • Issue: 2

Comparison of the antimicrobial efficacy of positive pressure and negative pressure irrigation technioues using chlorhexidine on entrococcus faecalis: An in-vitro study

  • Author:
  • Gaurav Singla1, Tarun Kalra2, 3
  • Total Page Count: 5
  • Page Number: 7 to 11

1Lecturer, Deptt. of Conservative Dentistry and Endodontics, B.R.S. Dental College, Sultanpur, Panchkula

2Post Graduate student, Deptt. of Conservative Dentistry and Endodontics, B.R.S. Dental College, Sultanpur, Panchkula

3Reader, Deptt. of Conservative Dentistry and Endodontics, B.R.S. Dental College, Sultanpur, Panchkula

Online published on 20 September, 2012.

Abstract

The aim of this in vitro study was to determine whether irrigation with negative pressure was more etfective than traditional positive pressure in eradicating Enterococcus faecars using chlorhexidine.

Thirty, extracted mandibular premolars were sterilized and inoculated with E. faecalis for7 days. Specimens in the negative pressure group were irrigated using endovac system, whereas those in the positive pressure group were irrigated with 27-G needle using 2% chlorhexidine. With the help of sterile H-File dentinal shavings were collected from the irrigated specimen. Dentinal shavings were dipped into sterile brain heart infusion broth and incubated for 48 hrs at 37°C. The occurrence of broth turbidity was indicative of bacteria remaining in the root canal.

Results were statistically significant when comparing apical negative-pressure irrigation to positive-pressure irrigation (p=0.020).

The results of this in vitro study showed that apical negative-pressure irrigation has the potential to achieve better microbial control than traditional irrigation delivery systems.

Keywords

Endovac, Enterococcus Faecalis, Chlorhexidine