Simultaneous Determination of Dexibuprofen and Tramadol HCl by HPTLC Method

Aim of the study is to develop simple, specific, accurate HPTLC method for simultaneous determination of the Dexibuprofen and Tramadol HCl in pharmaceutical dosage form.

Stationary phase was precoated silica gel 60 F254. The Mobile Phase used was mixture of Chloroform: toluene: ethanol: glacial acetic acid (8: 4: 1: 0.5 v/v/v/v). The detection of the spots was carried out at 264nm and 271nm for Dexibuprofen and Tramadol HCl respectively.

The method was validated in terms of linearity, accuracy, precision and specificity. The Limit of detection for Dexibuprofen and Tramadol HCl was found to be 1μg and 0. 2 μg respectively. The Limit of quantification for Dexibuprofen and Tramadol HCl was found to be 1.6μg and 0.4μg respectively.

The method is free from the interferences due to excipients present in the formulation and can be successfully used for determine drug in pharmaceutical dosage form.