A RP-HPLC method for the simultaneous assay of amlodipine and hydrochlorthiazide in combined dosage form

This is the present work which is a new method development and validation was carried out for the estimation of Amlodipine besylate and hydrochlorthiazide by RP-HPLC technique. The wavelength selection was made at 245 nm in various literature were having a maximum absorbance at around 240 to 250 nm. Hence the wavelength was selected at 245 nm for the detection of the compound. In this method development several trials had been carried out and reported. Leading this to the optimized chromatographic conditions for the estimation of Amlodipine besylate and hydro chlorthiazide pharmaceutical dosage form. Preliminary studies involved trying C18, columns and several mobile phase compositions for the effective separation of these drugs by using symmetry C18Hypersil-BDS column eluted with the mobile phase Degassed water and acetonitile with a flow rate of 1.0ml/min and a detection wavelength of 245 nm with injection volume of 20 ml at ambient (25°C) temperature yielded the good results.