UV-Visible Spectrophotometric Determination of Azathioprine in Pharmaceutical Formulations Based on Oxidative coupling reaction with MBTH

A simple, sensitive, selective precise and accurate spectrophotometric method has been developed for the determination azathioprine in pure form, pharmaceutical formulations and blood sample by MBTH reagent. Spectrophotometric method was developed for determination of azathioprine at P^H-4.0 which is extractable at 620 nm. Beer's law is obeyed in the concentration ranges 10–60 μg ml⁻¹ for formulations and 4–24 μg ml⁻¹ for blood sample. %R.S.D was found to be 0.1933% & 0.2901 and Recovery 99.65% & 99.16% respectively. The result obtained in this method would have a great value when applied in quality control studies in laboratories. Any ingredients and excipients were not observed during the study. The developed method was validated with respect to accuracy, precision, and Specificity and Selectivity.