1N.R.C. on DNA Fingerprinting, National Bureau of Plant Genetic Resources, New Delhi 110 012.
2Botany Department, Kenyatta University, P.O. Box 43844, Nairobi.
3Tea Research Foundation, P.O. Box 820, Kericho.
Institute for Biotechnology Research, Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000, Nairobi.
Optimization of the reaction conditions for Polymerase Chain Reaction (PCR) should be done before the actual Random Amplified Polymorphic DNA (RAPD) analysis is carried out to get repeatable results. Optimizations of concentration was carried out the reaction components; template DNA and Taq DNA Polymerase. The optimum concentrations were determined by the ability to form amplification products with the highest consistency. The significance of optimization is discussed and the results compared with similar published ones.
Musa, optimization, banana, plantain, PCR
