Molecular Biology Laboratory, Department of Botany, University of Calcutta, 35, B. C. Road, Calcutta 700019 (West Bengal).
1Present address: Department of Agricultural Botany, Ch. Charan Singh University, Meerut 250 004 (Uttar Pradesh).
A study on genetic variability in indica rice landraces at both the protein and DNA levels was made using isozyme and Site Specific Putative Transposon Sequence Amplification (SSPTSA) analyses. The results showed that the level of polymorphism between the two methods varied widely, ranging from only 12.38% (isozymes) to 46.66% (SSPTSA). Genetic dissimilarity matrix between the two methods, tested by the Spearman Rank Correlation analysis, indicated a moderate correlation between isozymes and SSPTSA (rs = 0.36). It was concluded that the DNA based markers (SSPTSA) will be more useful than isozyme markers in detecting genetic diversity among the indica landraces of West Bengal.
Rice landraces, isozymes, plant transposon, genoryping, genetic diversiry
