In Vitro Conservation of Gentiana kurroo Royle: An Indigenous Threatened Medicinal Plant

In vitro conservation of shoot cultures of Gelltiana kurroo Royle, a threatened medicinal plant, was achieved. On a standard shoot culture medium, cultures could be successfully maintained for seven months at 25"C by replacing cotton plugs with polypropylene caps as enclosures for culture tubes. Low temperature storage at 4°C. successfully extended shelf life of cultures for over 30 months. The cultures grew normally on multiplication medium.