1Directorate of Wheat Research, Karnal (Haryana).
2NRC on DNA Fingerprinting, National Bureau of Plant Genetic Resources, New Delhi-110 012.
3Divsion of Seed Science and Technology, Indian Agriculture Research Institute, New Delhi-110 012.
Genetic variability among 26 released varieties/local cultivars of Triticum durum was estimated by RAPD marker analysis and the results discussed in view of their centre of breeding and the pedigree. A total of 4 series of primers were used to screen the polymorphic primers and the profiles generated by 15 of such types were used for analysis. The primers OPA3 and OPP6 were the most polymorphic primers and can be used in conjugation with the morphological markers for the identification of the varieties. The varieties developed by the selection among the local race Bansi were grouped together. The highest degree of polymorphism was between the local landrace Malvaraj with Motia and Jay. The clustering on the basis of RAPD markers in the present study was clearly on the pattern of their parentage. The varieties developed through the selection in the CIMMYT material were widely dispersed and therefore can be used along with the locallandraces in the breeding programme to diversify the genetic base. The grouping of the germplasm particularly the landraces by RAPD markers can be used in developing the Indian durum wheat cultivars with wider genetic base.
Diversity, Genetic Similarity, RAPD Markers, Triticum turgidum (durum)
