Indian Journal of Plant Genetic Resources

  • Year: 2005
  • Volume: 18
  • Issue: 3

Genetic Diversity Analysis of Field Bean (Lablab purpureus L.) Sweet Through RAPD Markers

  • Author:
  • BN Gnanesh, M Reddi Sekhar, K Gopal, K Raja Reddy, NP Eswara Reddy
  • Total Page Count: 3
  • DOI:
  • Page Number: 233 to 235

Department of Genetics and Plant Breeding, SV Agricultural College, Tzrupati, 517 502 (Andhra Pradesh).

Abstract

RAPD analysis based on six random primers detected a high level of genetic variation among the 15 field bean genotypes of southern India. A total of 46 bands were generated of which 42 were polymorphic. A high degree of polymorphism was obtained with the primers OPM 20 (11 bands), OPM I (9 bands) and OPM 3 (9 bands). The similarity index values ranged from 0.286 to 0.897 indicating a wide range of genetic diversity. The dendrogram consisted of four close-knit groups having 6, 5, 2 and 2 genotypes in groups 1, 2, 3 and 4, respectively. Based on RAPD marker analysis, MAC8, GA 100 and and GA 74-4 were found-to be diverse when compared to other genotypes.

Keywords

Field bean, Lablab purpureus (L.) Sweet, Clustering, Diversity, RAPD markers