A total of 74 released varieties of field crops (15 in wheat, 9 in maize, 19 in soybean, 6 in mung, 7 in md, 8 in lentil and 10 in gram) at Pantnagar, Uttarakhand were characterized using SDS-PAGE (sodium dodccyl sulphate polyacrylamide gel electrophoresis) of seed protein extracted in Tris-HCI buffer with the objective of finding certain critical bands which could be used as distinguishable features. Protein profiles of different field crops showed the number of bands ranging from 11 (urd, lentil and gram) to 16 (maize). Wheat and soybean displayed 12 bands each and mung showed 14 bands. It was possible to distinguish UP 2565 vs C 306, PBW 343, PBW 396, PBW 262, UP 1109, UP 2338. UP 2382 and UP 2425 vs Raj 3765, HD 2687, PBW 373, PBW 502 and UP 21 J3 vs PBW 175 in wheat; Pant Mung l and Pant Mung 1 from Pant Mung 3, Pant Mung 4, Pant Mung 5 and UPM 0205 in mung; Pant U 30, Pant U 35 ancl UPU 0031 from Manikya, UPU 97–10, Narendra Urd-1 and Pant U 19 in urcl; PL 4 and PL 406 from PL 02, PL 5, PL 018, PL 023, PL 234 and PL 639 in lentil and PG 118 from PGK 23, PG K 24, PG 033, PG 034, PG 035, PG 036, PG 037, PG 038 and Pant G 114 in gram on the basis of protein profiles. SDS-PAGE could not distinguish 19 varieties ofsoybean 9 varieties of maize. These critical bands could serve as additional descriptors in DUS testing.
SDS-PAGE, Field crops
