Use of Tissue Culture Technology in Vanilla and Possibilities of Germplasm Conservation
Abstract
Plantlets were produced from tip cultures of young aerial roots of Vanilla planifolia. With the breakdown of the root cap, the cells of the quiescent centre divided forming a hemispherical mass of cells which developed either into a single shoot meristem or organized themselves into several meristems bearing leaf primordia. After a few leaves have been formed a root meristem differentiated establishing a well formed plantlet. Since the constituent cells of the root apical meristems are genetically stable, less differentiated and permit plant regeneration in high frequency, they form an ideal material for the long-term preservation of germplasm.
