Department of Genetics and Plant Breeding, GB Pant University of Agriculture and Technology, Pantnagar-263145, Uttarakhand, India.
* Present address: Department of Genetics and Plant Breeding, SVB Patel University of Agriculture and Technology, Meerut-250110, (Uttar Pradesh) E-mail: gangwarlk@gmail.com
Electrophoretic analysis of seed protein of nine F4 progenies of three interspecific hybrids and their parents was carried out to study the variability in protein band expression. In all, 23 protein bands were identified ranging with molecular weight of 98 to 16 kD. Of these, three protein bands of 95.0, 71.5 and 60.0 kD were unique to Cajanus cajan, while four bands of 77.5, 67.5, 29.5 and 22.5 kD were specific to hybrids only. On the other hand C. cajanifolius has no unique protein band. Parents showed variability in protein band expression ranging from 7 in ICPL 84023 to 9 in UPAS 120 and Cajanus cajanifolius. The inter-progeny differences were observed for number, length and intensity of polypeptide bands of the protein. The similarity index of hybrids with Cajanus cajanifolius ranged from 35.29 to 66.66%. In general, bands at 87.5, 74.0, 46.0 and 34.5 kD were common in parents as well as hybrids.
Cajanus cajan, Cajanus cajanifolius, Electrophoresis, Interspecific hybrids, Pigeonpea, Seed protein
