1Department of Biotechnology, Dr YS Parmar University of Horticulture and Forestry, Nauni, Solan-173230, Himachal Pradesh, India.
2Seed Technology and Production Centre, Dr YS Parmar University of Horticulture and Forestry, Nauni, Solan-173230, Himachal Pradesh, India.
3Department of Floriculture and Landscaping, Dr YS Parmar University of Horticulture and Forestry, Nauni, Solan-173230, Himachal Pradesh, India.
*Author for Correspondence: E-mail: shirkotcp@yahoo.co.in
Woodfordia fruticosa (L.) Kurz (Lythraceae) is a rare woody shrub, the dried flowers of which are used as an important drug to treat various human disorders. Genetic variation of thirty genotypes of W. fruticosa was investigated using nine enzyme systems. Isozyme analysis of eight enzymes revealed considerable and very useful polymorphism among thirty genotypes from ten sites of Himachal Pradesh. Allele frequency in the thirty genotypes ranged from 0.5 to 1.00 and at all polymorphic loci only two alleles could be identified. On the basis of dendrogram the thirty genotypes were separated into two clusters. Cluster I included genotypes from Mandi, Hamirpur and Bilaspur districts, whereas Cluster II included genotypes from Solan and Sirmour districts. Polymorphism in the selected genotypes was found to be 34.5 per cent.
Woodfordia fruticosa, Genetic markers, Isozymes, Polyacrylamide gel electrophoresis
