1Department of Genetics and Plant Breeding, College of Horticulture, Andhra Pradesh Horticultural University, 1st floor of SBH, H.No.15–157, Kurnool Road, Kothakota (Post)-509 381, Mahaboobnagar, Andhra Pradesh.
2Cropping Systems Initiative for South Asia (CSISA) Sponsored by IRRI, Barwale Research Foundation-IRRI–Asia Liaison Office, Banjara Hills, Hyderabad–500 031, Andhra Pradesh.
Crop Improvement Division, Directorate of Rice Research, Rajendranagar, Hyderabad–500 030, Andhra Pradesh.
* Author for Correspondence: E-mail: saidu_genetics@yahoo.co.in
SSR markers targeting (GATA)n motifs are known to be highly polymorphic and useful in many organisms. Twenty eight germplasm of rice (Oryza sativa L.) involved as parents of some of the released hybrids were subjected to polymorphism based on JGT-SSRs analyses to asses the prevalence of genetic diversity. A total of 14 primers of SSRs amplified 37 polymorphic loci. The polymorphic information content ranged from 0.535 (JGT12-8.6) to 0.929 (JGT1–16.2). Prevalence of genetic diversity was revealed by (GATA)n SSRs as range of similarity coefficient value of 0.31 to 1. The parents were grouped by subjecting the similarity values to UPGMA based SAHN clustering. Study revealed the existence of sufficient genetic variation at DNA level in the genotypes being used as parental lines. The results suggested that a relatively less number of (GATA)n microsatellite markers can be used for the reliable estimation of genetic diversity and identification of duplicates.
Genotypes, JGT-SSRs, Molecular diversity, Rice
