Indian Journal of Plant Genetic Resources
  • Year: 2011
  • Volume: 24
  • Issue: 2

Seed Storage Protein Profi les of Pea (Pisum sativum L.) Genotypes using SDS-PAGE

  • Author:
  • Deepali Pandey1,, YV Singh2
  • Total Page Count: 11
  • Page Number: 194 to 204

1Krishi Vigyan Kendra, Kashipur, US Nagar, Uttarakhand

2Department of Vegetable Science, GB Pant University of Agriculture and Technology, Pantnagar-263 145, Uttarakhand

*Author for Correspondence: E-mail: deepalipan@gmail.com

Abstract

Using polyacrylamide slab gel electrophoresis, the water soluble seed proteins could be resolved in 42 bands distributed in six zones. One hundred and thirteen cultivars were categorized into 28 groups; each group had distinct and different electropherotic banding patterns. However, within the group cultivars were also further distinguishable by the intensity of staining of bands. It was possible through electrophoresis to differentiate phenotypically similar cultivars for example UU-12, Multifreezer, Piourette, Starcovert, Bonneville and IP-3. Cultivar Meethi Phali with a specific characteristic of edible pod type formed a separate group with 9 bands. Most of the varieties showed no distinct patterns which would allow their characterization. There were a few specific bands in each variety and most of them; although exclusive, appeared at such a low frequency that varietal characterization was not possible.

Keywords

Electropherogram, Genotypes, Pea, SDS-PAGE, Seed protein